Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

[KO Validated] Cyclin D1 Rabbit mAb (A19038)

KO/KDValidated

Publications (100) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of lysates from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of various lysates using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (A19038, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

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Overview

Product name [KO Validated] Cyclin D1 Rabbit mAb
Catalog No. A19038
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0300

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).
Sequence ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Gene ID 595
Swiss prot P24385
Synonyms BCL1; PRAD1; U21B31; D11S287E; D1
Calculated MW 34kDa
Observed MW 34kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanMouseRat
WB HumanMouse
IF/ICC Mouse
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HepG2 , MCF7 , HeLa , Mouse lung
Cellular location Nucleus
Customer validation

WB (Homo sapiens, Mus musculus, Rattus norvegicus, Homo sapeins)

IHC (Mus musculus)

IF (Mus musculus, Rattus norvegicus, Homo sapiens)

RT-qPCR (Homo sapiens)

IB (Homo sapiens)

WBIHC (Homo sapiens)

Co-IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Cyclin D1 Rabbit mAb images

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)}

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of lysates from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)}

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of various lysates using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)}

Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit mAb (A19038, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCND1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCND1. (Distance between topics and target gene indicate popularity.) CCND1

* Data provided by citexs.com, for reference only.

Publishing research using A19038? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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