Products > Catalog Antibodies > Monoclonal Antibodies

[KO Validated] Cyclin D1 Rabbit mAb (A19038)

Publications (20) Datasheet

ABclonal:[KO Validated] Cyclin D1 Rabbit mAb
ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody (A19038). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (A19038) at a dilition of 1:1000.

Overview

Product name[KO Validated] Cyclin D1 Rabbit mAb
Catalog No.A19038
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human Cyclin D1
SequenceEmail for sequence
Gene ID595
Swiss protP24385
SynonymsBCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; cyclin D1
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHepG2, MCF7, Mouse lung, HeLa
Cellular locationCytoplasm, Membrane, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Cyclin D1 Rabbit mAb (A19038) }

Immunoprecipitation - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody (A19038). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (A19038) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A19038 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A19038? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order