Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation - [KO Validated] Cyclin D1 Rabbit mAb (A19038)
Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody (A19038). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (A19038) at a dilition of 1:1000.