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[KO Validated] Cyclin D1 Rabbit mAb (A19038)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A19038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody (A19038). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (A19038) at a dilition of 1:1000.

Overview

Product name	[KO Validated] Cyclin D1 Rabbit mAb
Catalog No.	A19038
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).
Sequence	ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Gene ID	595
Swiss prot	P24385
Synonyms	BCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; cyclin D1
Calculated MW	34kDa
Observed MW	34kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HepG2, MCF7, Mouse lung, HeLa
Cellular location	Cytoplasm, Membrane, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus)

Research Area

[KO Validated] Cyclin D1 Rabbit mAb images

Western blot - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunohistochemistry - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product Protocols

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References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Technol Cancer Res Treat

Application:

IF:

3.399

Species:

Homo sapiens

PMID:

32885717

Title:

Downregulation of miR-499a-5p Predicts a Poor Prognosis of Patients With Non-Small Cell Lung Cancer and Restrains the Tumorigenesis by Targeting Fibroblast Growth Factor 9

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Oncol Lett

Application:

IF:

2.967

Species:

Homo sapiens

PMID:

33240422

Title:

TERT promoter regulating melittin expression induces apoptosis and G 0/G 1 cell cycle arrest in esophageal carcinoma cells

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Sci Adv

Application:

IF:

14.136

Species:

Homo sapiens

PMID:

33239293

Title:

Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Theranostics

Application:

IF:

11.556

Species:

Homo sapiens

PMID:

33456583

Title:

Cytoplasmic SHMT2 drives the progression and metastasis of colorectal cancer by inhibiting β-catenin degradation

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Acta Trop

Application:

IF:

3.11

Species:

Mus musculus

PMID:

34389327

Title:

Microcyst fluid promotes the migration and invasion of fibroblasts in the adventitial layer of alveolar echinococcosis

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Cell Death Dis

Application:

IF:

8.46

Species:

Homo sapiens

PMID:

33824311

Title:

MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

J Pharmacol Sci

Application:

IF:

3.33

Species:

Mus musculus

PMID:

34507635

Title:

Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Vet Microbiol

Application:

IF:

3.29

Species:

Mus musculus

PMID:

34649012

Title:

Next-generation sequencing sheds light on the interaction between virus and cell during foot-and-mouth disease virus persistent infection

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Acta Biochim Biophys Sin (Shanghai)

Application:

IF:

3.848

Species:

Homo sapiens

PMID:

34718369

Title:

PAQR4 promotes the development of hepatocellular carcinoma by activating PI3K/AKT pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

34111425

Title:

Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP Inhibitor in multiple myeloma

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Biomed Pharmacother

Application:

IF:

6.529

Species:

Homo sapiens

PMID:

34148735

Title:

Isothiocyanate Iberin inhibits cell proliferation and induces cell apoptosis in the progression of ovarian cancer by mediating ROS accumulation and GPX1 expression

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Neoplasia

Application:

IF:

5.715

Species:

Homo sapiens

PMID:

34325342

Title:

PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

J Transl Med

Application:

IF:

5.531

Species:

Homo sapiens

PMID:

34627268

Title:

CENPA promotes clear cell renal cell carcinoma progression and metastasis via Wnt/β-catenin signaling pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

3 Biotech

Application:

IF:

2.406

Species:

Homo sapiens

PMID:

34458056

Title:

Tumor-promoting function of PIMREG in glioma by activating the β-catenin pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

J Ethnopharmacol

Application:

IF:

4.36

Species:

Rattus norvegicus

PMID:

34648900

Title:

Pretreatment with the active fraction of Rhodiola tangutica (Maxim.) SH Fu rescues hypoxia-induced potassium channel inhibition in rat pulmonary artery smooth muscle cells

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Balkan Med J

Application:

IF:

Species:

Homo sapiens

PMID:

34860160

Title:

Knockdown of Serum- and Glucocorticoid-Regulated Kinase 1 Enhances Cisplatin Sensitivity of Gastric Cancer Through Suppressing the Nuclear Factor Kappa-B Signaling Pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Exp Cell Res

Application:

IF:

3.905

Species:

Homo sapiens

PMID:

34780782

Title:

CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Bioengineered

Application:

IF:

3.269

Species:

Homo sapiens

PMID:

34823423

Title:

Ubiquitin-associated protein 2 like (UBAP2L) enhances growth and metastasis of gastric cancer cells

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

In Vitro Cell Dev Biol Anim

Application:

IF:

2.416

Species:

Homo sapiens

PMID:

34888748

Title:

Nucleoporin 37 promotes the cell proliferation, migration, and invasion of gastric cancer through activating the PI3K/AKT/mTOR signaling pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Mus musculus

PMID:

34867383

Title:

Network Pharmacology and Molecular Docking-Based Investigation: Prunus mume Against Colorectal Cancer via Silencing RelA Expression

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

J Agric Food Chem

Application:

IF:

5.279

Species:

Mus musculus

PMID:

34928143

Title:

Characterizations and the Mechanism Underlying Osteogenic Activity of Peptides from Enzymatic Hydrolysates of Stichopus japonicus

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Cellular signalling

Application:

IF:

4.31

Species:

Homo sapiens

PMID:

34229087

Title:

NPR3, transcriptionally regulated by POU2F1, inhibits osteosarcoma cell growth through blocking the PI3K/AKT pathway

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Carcinogenesis

Application:

IF:

4.94

Species:

Homo sapiens

PMID:

30239617

Title:

G1 phase cell cycle arrest in NSCLC in response to LZ-106, an analog of enoxacin, is orchestrated through ROS overproduction in a P53-dependent manner

References for [KO Validated] Cyclin D1 Rabbit mAb (A19038)

Product:

[KO Validated] Cyclin D1 Rabbit mAb

Journal:

Digestive diseases and sciences

Application:

IF:

Species:

Homo sapiens

PMID:

34313922

Title: