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[KO Validated] β-Catenin Rabbit mAb (A19657)

Review (1)Publications (17) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of HeLa cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of Mouse brain, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts from wild type (WT) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using β-Catenin antibody (A19657) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human small cell lung cancer using β-Catenin antibody (A19657) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human breast cancer using β-Catenin antibody (A19657) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunofluorescence analysis of C6 cells using β-Catenin antibody (A19657) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug β-Catenin antibody (A19657). Western blot was performed from the immunoprecipitate using β-Catenin (A19657) at a dilition of 1:1000.

Review
ABclonal: review for [KO Validated] β-Catenin Rabbit mAb(A19657)

Overview

Product name[KO Validated] β-Catenin Rabbit mAb
Catalog No.A19657
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (P35222).
SequenceMATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Gene ID1499
Swiss protP35222
SynonymsCTNNB; MRD19; armadillo; beta Catenin; CTNNB1
Calculated MW92kDa
Observed MW92kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Mouse brain, 293T
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

(正常小鼠脑部海马体)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens)

IF(Mus musculus, Homo sapiens)

IF(Homo sapiens, Mus musculus)

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of HeLa cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of Mouse brain, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts from wild type (WT) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using β-Catenin antibody (A19657) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human small cell lung cancer using β-Catenin antibody (A19657) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human breast cancer using β-Catenin antibody (A19657) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunofluorescence analysis of C6 cells using β-Catenin antibody (A19657) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)}

Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug β-Catenin antibody (A19657). Western blot was performed from the immunoprecipitate using β-Catenin (A19657) at a dilition of 1:1000.

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