Products > Catalog Antibodies > Monoclonal Antibodies

[KO Validated] β-Catenin Rabbit mAb (A19657)

Review (1)Publications (8) Datasheet

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of HeLa cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of Mouse brain, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunofluorescence analysis of C6 cells using β-Catenin antibody (A19657) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug β-Catenin antibody (A19657). Western blot was performed from the immunoprecipitate using β-Catenin (A19657) at a dilition of 1:1000.

Review
ABclonal: review for [KO Validated] β-Catenin Rabbit mAb(A19657)

Overview

Product name[KO Validated] β-Catenin Rabbit mAb
Catalog No.A19657
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
ImmunogenA synthesized peptide derived from human beta Catenin.
SequenceEmail for sequence
Gene ID1499
Swiss protP35222
SynonymsCTNNB; MRD19; armadillo; beta Catenin; CTNNB1
Calculated MW92kDa
Observed MW92kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Mouse brain, 293T
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation

(正常小鼠脑部海马体)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of HeLa cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of Mouse brain, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Western blot - [KO Validated] β-Catenin Rabbit mAb (A19657)

Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] β-Catenin Rabbit mAb (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunofluorescence - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunofluorescence analysis of C6 cells using β-Catenin antibody (A19657) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657) }

Immunoprecipitation - [KO Validated] β-Catenin Rabbit mAb (A19657)

Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug β-Catenin antibody (A19657). Western blot was performed from the immunoprecipitate using β-Catenin (A19657) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A19657

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A19657 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A19657? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order