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[KO Validated] Caspase-8 Rabbit mAb (A19549)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] Caspase-8 Rabbit mAb (A19549)

Western blot analysis of extracts from normal (control) and Caspase-8 knockout (KO) HeLa cells, using Caspase-8 antibody (A19549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] Caspase-8 Rabbit mAb (A19549)

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (A19549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product name[KO Validated] Caspase-8 Rabbit mAb
Catalog No.A19549
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human Caspase-8.
SequenceEmail for sequence
Gene ID841
Swiss protQ14790
SynonymsALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; Caspase 8; CASP8; Caspase-8; caspase-8
Calculated MW55kDa
Observed MW55KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesJurkat, HepG2, HeLa
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] Caspase-8 Rabbit mAb (A19549) }

Western blot - [KO Validated] Caspase-8 Rabbit mAb (A19549)

Western blot analysis of extracts from normal (control) and Caspase-8 knockout (KO) HeLa cells, using Caspase-8 antibody (A19549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] Caspase-8 Rabbit mAb (A19549) }

Western blot - [KO Validated] Caspase-8 Rabbit mAb (A19549)

Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (A19549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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