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[KO Validated] Caspase-3 Rabbit pAb (A2156)

Reviews (6)Publications (45) Datasheet

ABclonal:Western blot - [KO Validated] Caspase-3 Rabbit pAb (A2156)
ABclonal:Western blot - [KO Validated] Caspase-3 Rabbit pAb (A2156)
ABclonal:Immunohistochemistry - [KO Validated] Caspase-3 Rabbit pAb (A2156)
ABclonal:Immunohistochemistry - [KO Validated] Caspase-3 Rabbit pAb (A2156)
Review
ABclonal: review for [KO Validated] Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for [KO Validated] Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for [KO Validated] Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for [KO Validated] Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for [KO Validated] Caspase-3 Rabbit pAb(A2156)
Review
ABclonal: review for [KO Validated] Caspase-3 Rabbit pAb(A2156)

Overview

Product name[KO Validated] Caspase-3 Rabbit pAb
Catalog No.A2156
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
SequenceFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Gene ID836
Swiss protP42574
SynonymsCPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3
Calculated MW31kDa
Observed MW17kDa, 35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesJurkat
Cellular locationCytoplasm
Customer validation

(Human uterine stromal cells, B6, Rat brain, NMuMG, Mouse kidney, HeLa)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Gallus gallus, Bos taurus, Panax notoginseng (Burk.) F. H. Chen ex C. Chow, Spodoptera frugiperda)

IHC(Mus musculus)

ABclonal:Western blot - [KO Validated] Caspase-3 Rabbit pAb (A2156) }

Western blot - [KO Validated] Caspase-3 Rabbit pAb (A2156)

Western blot analysis of extracts of Jurkat cells, using Caspase-3 antibody (A2156) at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - [KO Validated] Caspase-3 Rabbit pAb (A2156) }

Western blot - [KO Validated] Caspase-3 Rabbit pAb (A2156)

Western blot analysis of extracts from normal (control) and Caspase-3 knockout (KO) 293T cells, using Caspase-3 antibody (A2156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - [KO Validated] Caspase-3 Rabbit pAb (A2156) }

Immunohistochemistry - [KO Validated] Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry of paraffin-embedded rat spleen using Caspase-3 antibody (A2156) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Caspase-3 Rabbit pAb (A2156) }

Immunohistochemistry - [KO Validated] Caspase-3 Rabbit pAb (A2156)

Immunohistochemistry of paraffin-embedded human tonsil using Caspase-3 antibody (A2156) at dilution of 1:100 (40x lens).

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