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[KO Validated] CSPG4 Rabbit pAb (A3592)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts of A375 cells, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts of various cell lines, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] CSPG4 Rabbit pAb (A3592)

Immunofluorescence analysis of A-375 cells using [KO Validated] CSPG4 Rabbit pAb (A3592) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] CSPG4 Rabbit pAb
Catalog No.A3592
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).
SequenceLEVPQALGRSSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVA
Gene ID1464
Swiss protQ6UVK1
SynonymsCSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
Calculated MW250kDa
Observed MW250KDa-450KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A375, Mouse heart, Rat brain, Rat heart
Cellular locationApical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane
Customer validation

WB(Mus musculus)

IF(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592) }

Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts of A375 cells, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592) }

Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts of various cell lines, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592) }

Western blot - [KO Validated] CSPG4 Rabbit pAb (A3592)

Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (A3592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] CSPG4 Rabbit pAb (A3592) }

Immunofluorescence - [KO Validated] CSPG4 Rabbit pAb (A3592)

Immunofluorescence analysis of A-375 cells using [KO Validated] CSPG4 Rabbit pAb (A3592) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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