Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] CSNK1E Rabbit pAb (A1796)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] CSNK1E Polyclonal Antibody (A1796)
ABclonal:Western blot - [KO Validated] CSNK1E Polyclonal Antibody (A1796)
ABclonal:Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (A1796)
ABclonal:Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (A1796)
ABclonal:Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (A1796)

Overview

Product name[KO Validated] CSNK1E Rabbit pAb
Catalog No.A1796
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
SequenceEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK
Gene ID1454
Swiss protP49674
SynonymsCSNK1E; CKIepsilon; HCKIE
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - [KO Validated] CSNK1E Polyclonal Antibody (A1796) }

Western blot - [KO Validated] CSNK1E Polyclonal Antibody (A1796)

Western blot analysis of extracts of various cell lines, using CSNK1E antibody (A1796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CSNK1E Polyclonal Antibody (A1796) }

Western blot - [KO Validated] CSNK1E Polyclonal Antibody (A1796)

Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (A1796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (A1796) }

Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (A1796)

Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (A1796) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (A1796) }

Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (A1796)

Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (A1796) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (A1796) }

Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (A1796)

Immunofluorescence analysis of HeLa cells using CSNK1E antibody (A1796). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A1796 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1796? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order