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[KO Validated] CSK Rabbit pAb (A0735)

Datasheet

ABclonal:Western blot - [KO Validated] CSK Rabbit pAb (A0735)
ABclonal:Western blot - [KO Validated] CSK Rabbit pAb (A0735)
ABclonal:Western blot - [KO Validated] CSK Rabbit pAb (A0735)
ABclonal:Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735)
ABclonal:Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735)
ABclonal:Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735)

Overview

Product name[KO Validated] CSK Rabbit pAb
Catalog No.A0735
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 241-450 of human CSK (NP_004374.1).
SequenceTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL
Gene ID1445
Swiss protP41240
SynonymsCSK
Calculated MW50kDa
Observed MW50KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, Jurkat, Raji, Mouse lung, Mouse spleen, Mouse thymus, Rat lung
Cellular locationCell membrane, Cytoplasm
ABclonal:Western blot - [KO Validated] CSK Rabbit pAb (A0735) }

Western blot - [KO Validated] CSK Rabbit pAb (A0735)

Western blot analysis of extracts from normal (control) and CSK knockout (KO) 293T cells, using CSK antibody (A0735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] CSK Rabbit pAb (A0735) }

Western blot - [KO Validated] CSK Rabbit pAb (A0735)

Western blot analysis of extracts of various cell lines, using CSK antibody (A0735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] CSK Rabbit pAb (A0735) }

Western blot - [KO Validated] CSK Rabbit pAb (A0735)

Western blot analysis of extracts of various cell lines, using CSK antibody (A0735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735) }

Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735)

Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] CSK Rabbit pAb (A0735) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735) }

Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CSK Rabbit pAb (A0735) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735) }

Immunohistochemistry - [KO Validated] CSK Rabbit pAb (A0735)

Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CSK Rabbit pAb (A0735) at dilution of 1:100 (40x lens).

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