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[KO Validated] CRKL Rabbit pAb (A11735)

Datasheet

ABclonal:Western blot - [KO Validated] CRKL Polyclonal Antibody (A11735)
ABclonal:Western blot - [KO Validated] CRKL Polyclonal Antibody (A11735)

Overview

Product name[KO Validated] CRKL Rabbit pAb
Catalog No.A11735
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).
SequenceMSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE
Gene ID1399
Swiss protP46109
SynonymsCRKL
Calculated MW33kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesLO2, Jurkat, SKOV3, Mouse brain, Rat heart
Cellular location
ABclonal:Western blot - [KO Validated] CRKL Polyclonal Antibody (A11735) }

Western blot - [KO Validated] CRKL Polyclonal Antibody (A11735)

Western blot analysis of extracts of various cell lines, using CRKL Antibody (A11735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] CRKL Polyclonal Antibody (A11735) }

Western blot - [KO Validated] CRKL Polyclonal Antibody (A11735)

Western blot analysis of extracts from normal (control) and CRKL knockout (KO) HeLa cells, using CRKL antibody (A11735) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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