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[KO Validated] CHRAC1 Rabbit pAb (A14896)

Datasheet

ABclonal:Western blot - [KO Validated] CHRAC1 Polyclonal Antibody (A14896)

Overview

Product name[KO Validated] CHRAC1 Rabbit pAb
Catalog No.A14896
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human CHRAC1 (NP_059140.1).
SequenceMADVVVGKDKGGEQRLISLPLSRIRVIMKSSPEVSSINQEALVLTAKATELFVQCLATYSYRHGSGKEKKVLTYSDLANTAQQSETFQFLADILPKKILASKYLKMLKEEKREEDEENDNDNESDHDEADS
Gene ID54108
Swiss protQ9NRG0
SynonymsCHRAC1; CHARC1; CHARC15; CHRAC-1; CHRAC-15; CHRAC15; YCL1
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] CHRAC1 Polyclonal Antibody (A14896) }

Western blot - [KO Validated] CHRAC1 Polyclonal Antibody (A14896)

Western blot analysis of extracts from normal (control) and CHRAC1 knockout (KO) HeLa cells, using CHRAC1 antibody (A14896) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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