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[KO Validated] CHD1 Rabbit pAb (A7883)

Datasheet

ABclonal:Western blot - [KO Validated] CHD1 Polyclonal Antibody (A7883)
ABclonal:Western blot - [KO Validated] CHD1 Polyclonal Antibody (A7883)
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883)
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883)
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883)

Overview

Product name[KO Validated] CHD1 Rabbit pAb
Catalog No.A7883
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
SequenceDREKHRKLDDHRSRDHRSNLEGSLKDRSHSDHRSHSDHRLHSDHRSSSEYTHHKSSRDYRYHSDWQMDHRASSSGPRSPLDQRSPYGSRSPFEHSVEHKSTPEHTWSSRKT
Gene ID1105
Swiss protO14646
SynonymsCHD1
Calculated MW196kDa
Observed MW197kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Jurkat, K-562
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - [KO Validated] CHD1 Polyclonal Antibody (A7883) }

Western blot - [KO Validated] CHD1 Polyclonal Antibody (A7883)

Western blot analysis of extracts of various cell lines, using CHD1 Antibody (A7883) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CHD1 Polyclonal Antibody (A7883) }

Western blot - [KO Validated] CHD1 Polyclonal Antibody (A7883)

Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 antibody (A7883) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883) }

Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883)

Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody (A7883) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883) }

Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883)

Immunohistochemistry of paraffin-embedded human liver damage using CHD1 antibody (A7883) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883) }

Immunohistochemistry - [KO Validated] CHD1 Polyclonal Antibody (A7883)

Immunohistochemistry of paraffin-embedded rat brain using CHD1 antibody (A7883) at dilution of 1:100 (40x lens).

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