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[KO Validated] CDK8 Rabbit pAb (A5548)

Datasheet

ABclonal:Western blot - [KO Validated] CDK8 Polyclonal Antibody (A5548)
ABclonal:Western blot - [KO Validated] CDK8 Polyclonal Antibody (A5548)
ABclonal:Immunoprecipitation - [KO Validated] CDK8 Polyclonal Antibody (A5548)

Overview

Product name[KO Validated] CDK8 Rabbit pAb
Catalog No.A5548
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).
SequenceDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY
Gene ID1024
Swiss protP49336
SynonymsCDK8; K35
Calculated MW53kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesJurkat
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] CDK8 Polyclonal Antibody (A5548) }

Western blot - [KO Validated] CDK8 Polyclonal Antibody (A5548)

Western blot analysis of extracts of Jurkat cells, using CDK8 antibody (A5548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CDK8 Polyclonal Antibody (A5548) }

Western blot - [KO Validated] CDK8 Polyclonal Antibody (A5548)

Western blot analysis of extracts from normal (control) and CDK8 knockout (KO) HeLa cells, using CDK8 antibody (A5548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunoprecipitation - [KO Validated] CDK8 Polyclonal Antibody (A5548) }

Immunoprecipitation - [KO Validated] CDK8 Polyclonal Antibody (A5548)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK8 antibody (A5548). Western blot was performed from the immunoprecipitate using CDK8 antibody (A5548) at a dilition of 1:1000.

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