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[KO Validated] CDK6 Rabbit pAb (A1545)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - [KO Validated] CDK6 Polyclonal Antibody (A1545)
ABclonal:Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (A1545)
Review
ABclonal: review for [KO Validated] CDK6 Rabbit pAb(A1545)

Overview

Product name[KO Validated] CDK6 Rabbit pAb
Catalog No.A1545
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human CDK6 (NP_001250.1).
SequenceMEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA
Gene ID1021
Swiss protQ00534
SynonymsCDK6; MCPH12; PLSTIRE
Calculated MW36kDa
Observed MW37kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, Jurkat, K-562
Cellular locationCell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle
Customer validation

(HepG2)

WB(Mus musculus, Homo sapiens)

IF(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - [KO Validated] CDK6 Polyclonal Antibody (A1545) }

Western blot - [KO Validated] CDK6 Polyclonal Antibody (A1545)

Western blot analysis of extracts from normal (control) and CDK6 knockout (KO) HeLa cells, using CDK6 antibody (A1545) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (A1545) }

Immunoprecipitation - [KO Validated] CDK6 Polyclonal Antibody (A1545)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug CDK6 antibody (A1545). Western blot was performed from the immunoprecipitate using CDK6 antibody (A1545) at a dilition of 1:1000.

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