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CD44 Rabbit mAb (A19020)

Publications (2) Datasheet

ABclonal:CD44 Rabbit mAb
ABclonal:Western blot - CD44 Rabbit mAb (A19020)

Western blot analysis of extracts of U-251MG cells, using CD44 antibody (A19020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - CD44 Rabbit mAb (A19020)

Immunohistochemistry of paraffin-embedded human esophageal using CD44 Rabbit mAb (A19020) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameCD44 Rabbit mAb
Catalog No.A19020
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human CD44
SequenceEmail for sequence
Gene ID960
Swiss protP16070
SynonymsCDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44; CD44
Calculated MW82kDa
Observed MW82kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-251MG, HeLa
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - CD44 Rabbit mAb (A19020) }

Western blot - CD44 Rabbit mAb (A19020)

Western blot analysis of extracts of U-251MG cells, using CD44 antibody (A19020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - CD44 Rabbit mAb (A19020) }

Immunohistochemistry - CD44 Rabbit mAb (A19020)

Immunohistochemistry of paraffin-embedded human esophageal using CD44 Rabbit mAb (A19020) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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