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[KO Validated] CD44 Rabbit pAb (A12410)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Western blot - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Overview

Product name[KO Validated] CD44 Rabbit pAb
Catalog No.A12410
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 148-247 of human CD44 (NP_001189486.1).
SequenceVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATRDQDTFHPSGGSHTTHGSESDGHSH
Gene ID960
Swiss protP16070
SynonymsCDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44; CD44
Calculated MW3kDa/15kDa/32-53kDa/73-81kDa
Observed MW82kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

FC(Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - [KO Validated] CD44 Rabbit pAb (A12410) }

Western blot - [KO Validated] CD44 Rabbit pAb (A12410)

Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using CD44 antibody (A12410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] CD44 Rabbit pAb (A12410) }

Western blot - [KO Validated] CD44 Rabbit pAb (A12410)

Western blot analysis of extracts of mouse spleen, using CD44 Antibody (A12410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410) }

Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:250 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410) }

Immunohistochemistry - [KO Validated] CD44 Rabbit pAb (A12410)

Immunohistochemistry of paraffin-embedded human lung cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:250 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410) }

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Immunofluorescence analysis of HeLa cells using CD44 antibody (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410) }

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Confocal immunofluorescence analysis of A431 cells using CD44 antibody (A12410) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410) }

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Immunofluorescence analysis of RAW264.7 cells using CD44 antibody (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410) }

Immunofluorescence - [KO Validated] CD44 Rabbit pAb (A12410)

Confocal immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Polyclonal Antibody (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining.

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