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[KO Validated] Cyclin D3 Rabbit pAb (A0746)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] Cyclin D3 Rabbit pAb (A0746)
ABclonal:Western blot - [KO Validated] Cyclin D3 Rabbit pAb (A0746)

Overview

Product name[KO Validated] Cyclin D3 Rabbit pAb
Catalog No.A0746
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human Cyclin D3 (NP_001751.1).
SequenceSMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL
Gene ID896
Swiss protP30281
SynonymsCCND3; cyclin D3; Cyclin D3
Calculated MW9kDa/22kDa/24kDa/32kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, K-562, Mouse spleen
Cellular locationCytoplasm, Membrane, Nucleus
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] Cyclin D3 Rabbit pAb (A0746) }

Western blot - [KO Validated] Cyclin D3 Rabbit pAb (A0746)

Western blot analysis of extracts of various cell lines, using Cyclin D3 antibody (A0746) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] Cyclin D3 Rabbit pAb (A0746) }

Western blot - [KO Validated] Cyclin D3 Rabbit pAb (A0746)

Western blot analysis of extracts from normal (control) and Cyclin D3 knockout (KO) HeLa cells, using Cyclin D3 antibody (A0746) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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