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[KO Validated] CBX3 Rabbit pAb (A2248)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] CBX3 Rabbit pAb (A2248)
ABclonal:Western blot - [KO Validated] CBX3 Rabbit pAb (A2248)
ABclonal:Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248)
ABclonal:Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248)
ABclonal:Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248)
ABclonal:Immunofluorescence - [KO Validated] CBX3 Rabbit pAb (A2248)
ABclonal:Immunofluorescence - [KO Validated] CBX3 Rabbit pAb (A2248)

Overview

Product name[KO Validated] CBX3 Rabbit pAb
Catalog No.A2248
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human CBX3 (NP_009207.2).
SequenceMASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMFLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ
Gene ID11335
Swiss protQ13185
SynonymsCBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Calculated MW20kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, MCF-7, 293T, A-549, NCI-H460, Mouse brain, Mouse lung, Mouse spleen
Cellular locationNucleus
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - [KO Validated] CBX3 Rabbit pAb (A2248) }

Western blot - [KO Validated] CBX3 Rabbit pAb (A2248)

Western blot analysis of extracts of various cell lines, using CBX3 antibody (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] CBX3 Rabbit pAb (A2248) }

Western blot - [KO Validated] CBX3 Rabbit pAb (A2248)

Western blot analysis of extracts from normal (control) and CBX3 knockout (KO) 293T cells, using CBX3 antibody (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248) }

Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248)

Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] CBX3 Rabbit pAb (A2248) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248) }

Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] CBX3 Rabbit pAb (A2248) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248) }

Immunohistochemistry - [KO Validated] CBX3 Rabbit pAb (A2248)

Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] CBX3 Rabbit pAb (A2248) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] CBX3 Rabbit pAb (A2248) }

Immunofluorescence - [KO Validated] CBX3 Rabbit pAb (A2248)

Immunofluorescence analysis of C6 cells using CBX3 antibody (A2248) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] CBX3 Rabbit pAb (A2248) }

Immunofluorescence - [KO Validated] CBX3 Rabbit pAb (A2248)

Immunofluorescence analysis of U-2 OS cells using CBX3 antibody (A2248) at dilution of 1:100. Blue: DAPI for nuclear staining.

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