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[KO Validated] CAST Rabbit pAb (A7634)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] CAST Polyclonal Antibody (A7634)
ABclonal:Western blot - [KO Validated] CAST Polyclonal Antibody (A7634)

Overview

Product name[KO Validated] CAST Rabbit pAb
Catalog No.A7634
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
SequenceSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPS
Gene ID831
Swiss protP20810
SynonymsCAST; BS-17; PLACK
Calculated MW63kDa/71- 84kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesA-549, SW480, MCF-7, HepG2, Mouse lung, Rat liver
Cellular location
Customer validation

WB(Homo sapiens,Sus scrofa, Mus musculus)

ABclonal:Western blot - [KO Validated] CAST Polyclonal Antibody (A7634) }

Western blot - [KO Validated] CAST Polyclonal Antibody (A7634)

Western blot analysis of extracts of various cell lines, using CAST antibody (A7634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] CAST Polyclonal Antibody (A7634) }

Western blot - [KO Validated] CAST Polyclonal Antibody (A7634)

Western blot analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using CAST antibody (A7634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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