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[KO Validated] CAPN2 Polyclonal Antibody (A1861)

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ABclonal:Western blot - [KO Validated] CAPN2 Polyclonal Antibody (A1861)
ABclonal:Western blot - [KO Validated] CAPN2 Polyclonal Antibody (A1861)
ABclonal:Immunofluorescence - [KO Validated] CAPN2 Polyclonal Antibody (A1861)
Review
ABclonal: review for [KO Validated] CAPN2 Polyclonal Antibody(A1861)

Overview

Product name[KO Validated] CAPN2 Polyclonal Antibody
Catalog No.A1861
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).
SequenceSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLD
Gene ID824
Swiss protP17655
SynonymsCAPN2; CANP2; CANPL2; CANPml; mCANP; calpain 2
Calculated MW71kDa/79kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesH460, A-549, HeLa, Mouse kidney, Mouse lung
Cellular locationCell membrane, Cytoplasm
Customer validation

WB(Pig)

ABclonal:Western blot - [KO Validated] CAPN2 Polyclonal Antibody (A1861) }

Western blot - [KO Validated] CAPN2 Polyclonal Antibody (A1861)

Western blot analysis of extracts of various cell lines, using CAPN2 antibody (A1861) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] CAPN2 Polyclonal Antibody (A1861) }

Western blot - [KO Validated] CAPN2 Polyclonal Antibody (A1861)

Western blot analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using CAPN2 antibody (A1861) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] CAPN2 Polyclonal Antibody (A1861) }

Immunofluorescence - [KO Validated] CAPN2 Polyclonal Antibody (A1861)

Immunofluorescence analysis of U2OS cells using CAPN2 antibody (A1861) at dilution of 1:100. Blue: DAPI for nuclear staining.

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