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[KO Validated] CAPG Rabbit pAb (A7324)

Datasheet

ABclonal:Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324)
ABclonal:Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324)
ABclonal:Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324)
ABclonal:Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (A7324)
ABclonal:Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (A7324)

Overview

Product name[KO Validated] CAPG Rabbit pAb
Catalog No.A7324
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
SequenceMYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGHESPIFKQFFKDWK
Gene ID822
Swiss protP40121
SynonymsCAPG; AFCP; HEL-S-66; MCP
Calculated MW36kDa/38kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTHP-1, HL-60, Mouse lung, Mouse spleen, Rat heart, Rat lung
Cellular locationCytoplasm, Melanosome, Nucleus
ABclonal:Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324) }

Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (A7324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324) }

Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324)

Western blot analysis of extracts of various cell lines, using CAPG Antibody (A7324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324) }

Western blot - [KO Validated] CAPG Polyclonal Antibody (A7324)

Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (A7324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (A7324) }

Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (A7324)

Immunohistochemistry of paraffin-embedded human lung cancer using CAPG Antibody (A7324) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (A7324) }

Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (A7324)

Immunohistochemistry of paraffin-embedded human liver cancer using CAPG Antibody (A7324) at dilution of 1:100 (40x lens).

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