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Beclin 1 Rabbit pAb (A7353)

Reviews (6)Publications (64) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Beclin 1 Rabbit pAb
ABclonal:Immunoprecipitation - Beclin 1 Rabbit pAb (A7353)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (A7353). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (A7353) at a dilition of 1:1000.

Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
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ABclonal: review for Beclin 1 Rabbit pAb(A7353)
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ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)

Overview

Product nameBeclin 1 Rabbit pAb
Catalog No.A7353
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human Beclin 1 (NP_003757.1).
SequenceLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG
Gene ID8678
Swiss protQ14457
SynonymsATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1
Calculated MW51kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
  • IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse brain, C6
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane
Customer validation

(C2C12, A549,Mouse kidney, U2OS,Saos-2, Human Isikawa , NPCs)

WB(Mus musculus, Sus scrofa, Rattus norvegicus, Homo sapiens, Gallus gallus, Spodoptera frugiperda, Anatinae, Pollen, Juvenile tilapia )

IHC(Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

WB(Mus musculus)

IF(Sus scrofa, Mus musculus, Rattus norvegicus)

IF(Pollen, Mus musculus)

ABclonal:Immunoprecipitation - Beclin 1 Rabbit pAb (A7353)}

Immunoprecipitation - Beclin 1 Rabbit pAb (A7353)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (A7353). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (A7353) at a dilition of 1:1000.

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