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[KO Validated] Beclin 1 Rabbit pAb (A7353)

Reviews (6)Publications (16) Datasheet

ABclonal:Western blot - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

ABclonal:Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

ABclonal:Immunofluorescence - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

ABclonal:Immunoprecipitation - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Review

Overview

Product name	[KO Validated] Beclin 1 Rabbit pAb
Catalog No.	A7353
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Sequence	MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG
Gene ID	8678
Swiss prot	Q14457
Synonyms	ATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1
Calculated MW	51kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIPseq RIP FC ELISA MeDIP Nucleotide Array
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	U-87MG, HeLa, MCF7, Mouse spleen, Rat ovary, Rat lung
Cellular location	Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane
Customer validation	(C2C12, A549,Mouse kidney, U2OS,Saos-2, NPCs, Human Isikawa ) WB(Mus musculus, Sus scrofa, Rattus norvegicus, Homo sapiens) IHC(Mus musculus) IF(Homo sapiens) Co-IP(Homo sapiens) IHC(Mus musculus) WB(Mus musculus)

Research Area

ResearchCell Biology & Developmental BiologyAutophagyRegulator

[KO Validated] Beclin 1 Rabbit pAb images

Western blot - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (A7353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (A7353) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 antibody (A7353) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (A7353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (A7353). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (A7353) at a dilition of 1:1000.

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References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Biomed Research International

Application:

IF:

2.476

Species:

Mus musculus

PMID:

28706950

Title:

Effects of Cobalt Chloride, a Hypoxia-Mimetic Agent, on Autophagy and Atrophy in Skeletal C2C12 Myotubes

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Scientific reports

Application:

IF:

4.258

Species:

Sus scrofa

PMID:

29051589

Title:

Autophagy induces apoptosis and death of T lymphocytes in the spleen of pigs infected with CSFV

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Journal of Cellular Biochemistry

Application:

IF:

3.085

Species:

Mus musculus

PMID:

29345345

Title:

Autophagy inhibition promotes phagocytosis of macrophage and protects mice from methicillin‐resistant staphylococcus aureus pneumonia

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY

Application:

IF:

5.104

Species:

Rattus norvegicus

PMID:

29533930

Title:

Choline Inhibits Ischemia-Reperfusion-Induced Cardiomyocyte Autophagy in Rat Myocardium by Activating Akt/mTOR Signaling

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Hepatology (Baltimore, Md.)

Application:

WB, IHC

IF:

14.07

Species:

Mus musculus

PMID:

30180281

Title:

Hepatitis B Virus Induces Autophagy to Promote its Replication by the Axis of miR‐192‐3p‐XIAP via NF‐κB Signaling

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Life sciences

Application:

IF:

3.23

Species:

Sus scrofa

PMID:

30381247

Title:

Adipose-derived stem cells reduce liver oxidative stress and autophagy induced by ischemia-reperfusion and hepatectomy injury in swine.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Journal of virology

Application:

WB, IF, Co-IP

IF:

4.36

Species:

Homo sapiens

PMID:

30541828

Title:

Autophagy benefits the replication of influenza A virus in vitro.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Acta biomaterialia

Application:

IHC, WB

IF:

6.38

Species:

Mus musculus

PMID:

30654213

Title:

Doxorubicin-polyglycerol-nanodiamond composites stimulate glioblastoma cell immunogenicity through activation of autophagy.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Nanomedicine

Application:

WB, IHC

IF:

Species:

Mus musculus

PMID:

30676239

Title:

Doxorubicin conjugated with nanodiamonds and in free form commit glioblastoma cells to heterodromous fates.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

5.63

Species:

Homo sapiens

PMID:

30718471

Title:

Stress-induced precocious aging in PD-patient iPSC-derived NSCs may underlie the pathophysiology of Parkinson's disease.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

3.11

Species:

Rattus norvegicus

PMID:

30959368

Title:

Genipin protects rats against lipopolysaccharide-induced acute lung injury by reinforcing autophagy.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

2.55

Species:

Homo sapiens

PMID:

31023529

Title:

Sodium butyrate induces autophagic apoptosis of nasopharyngeal carcinoma cells by inhibiting AKT/mTOR signaling.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Apoptosis : an international journal on programmed cell death

Application:

IF:

4.02

Species:

Rattus norvegicus

PMID:

31111379

Title:

Berberine mitigates IL-21/IL-21R mediated autophagic influx in fibroblast-like synoviocytes and regulates Th17/Treg imbalance in rheumatoid arthritis.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Theriogenology

Application:

IF:

2.29

Species:

Sus scrofa

PMID:

31437668

Title:

Lysosomal dysfunction disturbs porcine oocyte maturation and developmental capacity by disorganizing chromosome/cytoskeleton and activating autophagy/apoptosis.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

Journal of cellular physiology

Application:

IF:

3.92

Species:

Rattus norvegicus

PMID:

30953350

Title:

Bone marrow mesenchymal stem cells-derived conditioned medium protects cardiomyocytes from hypoxia/reoxygenation-induced injury through Notch2/mTOR/autophagy signaling.

References for [KO Validated] Beclin 1 Rabbit pAb (A7353)

Product:

[KO Validated] Beclin 1 Rabbit pAb

Journal:

International Journal of Molecular Medicine

Application:

IF:

2.92

Species:

Mus musculus

PMID:

31638187

Title:

Redd1 protects against post‑infarction cardiac dysfunction by targeting apoptosis and autophagy

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[KO Validated] Beclin 1 Rabbit pAb (A7353)

Overview

Background

Immunogen information

Applications

Research Area

[KO Validated] Beclin 1 Rabbit pAb images

Submit an review for A7353

Reviews List

Product Protocols

Inquire About This Product