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[KO Validated] Beclin 1 Rabbit pAb (A7353)

Reviews (6)Publications (18) Datasheet

ABclonal:Western blot - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)
ABclonal:Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)
ABclonal:Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)
ABclonal:Immunofluorescence - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)
ABclonal:Immunoprecipitation - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)
Review
ABclonal: review for [KO Validated] Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for [KO Validated] Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for [KO Validated] Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for [KO Validated] Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for [KO Validated] Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for [KO Validated] Beclin 1 Rabbit pAb(A7353)

Overview

Product name[KO Validated] Beclin 1 Rabbit pAb
Catalog No.A7353
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
SequenceMEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG
Gene ID8678
Swiss protQ14457
SynonymsATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1
Calculated MW51kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, HeLa, MCF7, Mouse spleen, Rat ovary, Rat lung
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane
Customer validation

(C2C12, A549,Mouse kidney, U2OS,Saos-2, NPCs, Human Isikawa )

WB(Mus musculus, Sus scrofa, Rattus norvegicus, Homo sapiens)

IHC(Mus musculus)

IF(Homo sapiens)

Co-IP(Homo sapiens)

IHC(Mus musculus)

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] Beclin 1 Polyclonal Antibody (A7353) }

Western blot - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (A7353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353) }

Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (A7353) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353) }

Immunohistochemistry - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunohistochemistry of paraffin-embedded mouse kidney using Beclin 1 antibody (A7353) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Beclin 1 Polyclonal Antibody (A7353) }

Immunofluorescence - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (A7353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Beclin 1 Polyclonal Antibody (A7353) }

Immunoprecipitation - [KO Validated] Beclin 1 Polyclonal Antibody (A7353)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (A7353). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (A7353) at a dilition of 1:1000.

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