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[KO Validated] Bax Rabbit mAb (A19684)

Publications (21) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Bax Rabbit mAb (A19684)

Western blot analysis of extracts of various cell lines, using Bax antibody (A19684) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry of paraffin-embedded rat spleen using Bax antibody (A19684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Bax antibody (A19684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit mAb (A19684)

Immunofluorescence analysis of L929 cells using Bax antibody (A19684). Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] Bax Rabbit mAb
Catalog No.A19684
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (Q07812).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID581
Swiss protQ07812
SynonymsBCL2L4; BAX
Calculated MW21kDa
Observed MW21KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, HeLa, Mouse lung, RAW264.7, Rat testis, Rat lung, 293T
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer validation

WB(Mus musculus, Sanghuangporus vaninii, Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - [KO Validated] Bax Rabbit mAb (A19684)}

Western blot - [KO Validated] Bax Rabbit mAb (A19684)

Western blot analysis of extracts of various cell lines, using Bax antibody (A19684) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)}

Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry of paraffin-embedded rat spleen using Bax antibody (A19684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)}

Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry of paraffin-embedded human colon carcinoma using Bax antibody (A19684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit mAb (A19684)}

Immunofluorescence - [KO Validated] Bax Rabbit mAb (A19684)

Immunofluorescence analysis of L929 cells using Bax antibody (A19684). Blue: DAPI for nuclear staining.

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