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[KO Validated] Bcl10 Rabbit pAb (A1106)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] Bcl10 Rabbit pAb (A1106)
ABclonal:Western blot - [KO Validated] Bcl10 Rabbit pAb (A1106)

Overview

Product name[KO Validated] Bcl10 Rabbit pAb
Catalog No.A1106
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human Bcl10 (NP_003912.1).
SequenceMEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ
Gene ID8915
Swiss protO95999
SynonymsBCL10; CARMEN; CIPER; CLAP; IMD37; c-E10; mE10
Calculated MW26kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMCF-7, DU145, A-549, BT-474, Jurkat
Cellular locationCytoplasm, Membrane raft, perinuclear region
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] Bcl10 Rabbit pAb (A1106) }

Western blot - [KO Validated] Bcl10 Rabbit pAb (A1106)

Western blot analysis of extracts of various cell lines, using Bcl10 antibody (A1106) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] Bcl10 Rabbit pAb (A1106) }

Western blot - [KO Validated] Bcl10 Rabbit pAb (A1106)

Western blot analysis of extracts from normal (control) and Bcl10 knockout (KO) HeLa cells, using Bcl10 antibody (A1106) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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