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[KO Validated] BAZ1B Rabbit pAb (A9851)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] BAZ1B Rabbit pAb (A9851)
ABclonal:Western blot - [KO Validated] BAZ1B Rabbit pAb (A9851)
ABclonal:Immunohistochemistry - [KO Validated] BAZ1B Rabbit pAb (A9851)
ABclonal:Immunohistochemistry - [KO Validated] BAZ1B Rabbit pAb (A9851)
ABclonal:Immunofluorescence - [KO Validated] BAZ1B Rabbit pAb (A9851)

Overview

Product name[KO Validated] BAZ1B Rabbit pAb
Catalog No.A9851
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
SequenceMAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQEVAELLKEEFPAWYEKLVLEMVHHNTASLEKLVDTAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPLEKVDEEATEKKSDGACDSPSSDKENSSQIAQDHQKKETVVKEDEGRRESINDRARRSPRKLPTSLKKGERKWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEIVRYFIRHN
Gene ID9031
Swiss protQ9UIG0
SynonymsBAZ1B; WBSCR10; WBSCR9; WSTF
Calculated MW170kDa
Observed MW190kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSW480, BT-474, SKOV3, HeLa, HepG2, Mouse thymus
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] BAZ1B Rabbit pAb (A9851) }

Western blot - [KO Validated] BAZ1B Rabbit pAb (A9851)

Western blot analysis of extracts of various cell lines, using BAZ1B antibody (A9851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] BAZ1B Rabbit pAb (A9851) }

Western blot - [KO Validated] BAZ1B Rabbit pAb (A9851)

Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (A9851) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] BAZ1B Rabbit pAb (A9851) }

Immunohistochemistry - [KO Validated] BAZ1B Rabbit pAb (A9851)

Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (A9851) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] BAZ1B Rabbit pAb (A9851) }

Immunohistochemistry - [KO Validated] BAZ1B Rabbit pAb (A9851)

Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (A9851) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] BAZ1B Rabbit pAb (A9851) }

Immunofluorescence - [KO Validated] BAZ1B Rabbit pAb (A9851)

Immunofluorescence analysis of HeLa cells using BAZ1B antibody (A9851) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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