Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Publications (6) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Immunofluorescence analysis of C6 cells using Acetyl-HMGB1-K29 Rabbit pAb (A16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Immunofluorescence analysis of NIH-3T3 cells using Acetyl-HMGB1-K29 Rabbit pAb (A16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit pAb (A16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

You may also interested in:

Overview

Product name Acetyl-HMGB1-K29 Rabbit pAb
Catalog No. A16002
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Immunogen information

Immunogen A synthetic acetylated peptide around K29 of human HMGB1.
Sequence Email for sequence
Gene ID 3146
Swiss prot P09429
Synonyms HMG1; HMG3; HMG-1; SBP-1; Acetyl-HMGB1-K29
Calculated MW 25kDa
Observed MW 28kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293T
Cellular location Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted
Customer validation

WB (Rana sylvatica, Xenopus laevis, Mus musculus, Homo sapiens, mus musculus)

IF (Mus musculus)

ELISA (mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Acetyl-HMGB1-K29 Rabbit pAb images

ABclonal:Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)}

Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Immunofluorescence analysis of C6 cells using Acetyl-HMGB1-K29 Rabbit pAb (A16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)}

Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Immunofluorescence analysis of NIH-3T3 cells using Acetyl-HMGB1-K29 Rabbit pAb (A16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)}

Immunofluorescence - Acetyl-HMGB1-K29 Rabbit pAb (A16002)

Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit pAb (A16002) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A16002 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on HMGB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HMGB1. (Distance between topics and target gene indicate popularity.) HMGB1

* Data provided by citexs.com, for reference only.

Publishing research using A16002? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Proteins (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order