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[KO Validated] ATG5 Rabbit mAb (A19677)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts from wild type (WT) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry of paraffin-embedded human placenta using ATG5 antibody (A19677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (A19677). Western blot was performed from the immunoprecipitate using ATG5 (A19677) at a dilition of 1:1000.

Review

Overview

Product name	[KO Validated] ATG5 Rabbit mAb
Catalog No.	A19677
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0).
Sequence	MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNI
Gene ID	9474
Swiss prot	Q9H1Y0
Synonyms	ATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5
Calculated MW	22kDa/32kDa
Observed MW	55KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HeLa, SH-SY5Y, Mouse brain, Mouse lung, 293T
Cellular location	Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Customer validation	(293T、 Hela 、A549、HepG2 、 PC-3、HL-60、RPMI-8226、MLOT-4 ) WB(Spodoptera frugiperda, Mus musculus)

Research Area

ResearchCell Biology & Developmental BiologyAutophagyATGs

[KO Validated] ATG5 Rabbit mAb images

Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (A19677). Western blot was performed from the immunoprecipitate using ATG5 (A19677) at a dilition of 1:1000.