Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
| Product name | [KO Validated] ATG5 Rabbit mAb |
|---|---|
| Catalog No. | A19677 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthesized peptide derived from human ATG5. |
|---|---|
| Sequence | Email for sequence |
| Gene ID | 9474 |
| Swiss prot | Q9H1Y0 |
| Synonyms | ATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5 |
| Calculated MW | 55kDa |
| Observed MW | 55KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | HeLa, SH-SY5Y, Mouse brain, Mouse lung, 293T |
| Cellular location | |
| Customer validation | WB(Mus musculus, Spodoptera frugiperda) |
* For research use only. Not for therapeutic or diagnostic purposes.
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![ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)](/Uploads/Catalog/A19677/A19677_1.jpg?t=1571637891 "ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)")
![ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)](/Uploads/Catalog/A19677/A19677_2.jpg?t=1571637892 "ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)")
![ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)](/Uploads/Catalog/A19677/A19677_3.jpg?t=1571637895 "ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)")
