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[KO Validated] ATG5 Rabbit mAb (A19677)

Review (1)Publications (2) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Western blot analysis of extracts from wild type (WT) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry of paraffin-embedded human placenta using ATG5 antibody (A19677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (A19677). Western blot was performed from the immunoprecipitate using ATG5 (A19677) at a dilition of 1:1000.

Review
ABclonal: review for [KO Validated] ATG5 Rabbit mAb(A19677)

Overview

Product name[KO Validated] ATG5 Rabbit mAb
Catalog No.A19677
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0).
SequenceMTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNI
Gene ID9474
Swiss protQ9H1Y0
SynonymsATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5
Calculated MW22kDa/32kDa
Observed MW55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, SH-SY5Y, Mouse brain, Mouse lung, 293T
Cellular locationCytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Customer validation

(293T、 Hela 、A549、HepG2 、 PC-3、HL-60、RPMI-8226、MLOT-4 )

WB(Spodoptera frugiperda, Mus musculus)

ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)}

Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Western blot analysis of extracts from wild type (WT) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)}

Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)}

Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry of paraffin-embedded human placenta using ATG5 antibody (A19677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)}

Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (A19677). Western blot was performed from the immunoprecipitate using ATG5 (A19677) at a dilition of 1:1000.

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