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[KO Validated] ATG5 Rabbit mAb (A19677)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)
ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)
ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Overview

Product name[KO Validated] ATG5 Rabbit mAb
Catalog No.A19677
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
ImmunogenA synthesized peptide derived from human ATG5.
SequenceEmail for sequence
Gene ID9474
Swiss protQ9H1Y0
SynonymsATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5
Calculated MW55kDa
Observed MW55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, SH-SY5Y, Mouse brain, Mouse lung, 293T
Cellular location
Customer validation

WB(Homo sapiens, Gallus gallus)

ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677) }

Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677) }

Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677) }

Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry of paraffin-embedded human placenta using ATG5 antibody (A19677) at dilution of 1:100 (40x lens).

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