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[KO Validated] ARID2 Rabbit pAb (A8601)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] ARID2 Polyclonal Antibody (A8601)
ABclonal:Western blot - [KO Validated] ARID2 Polyclonal Antibody (A8601)

Overview

Product name[KO Validated] ARID2 Rabbit pAb
Catalog No.A8601
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1586-1835 of human ARID2 (NP_689854.2).
SequenceQNVVPQNTPMPPSPAVQVQGQPNSSQPSPFSGSSQPGDPMRKPGQNFMCLWQSCKKWFQTPSQVFYHAATEHGGKDVYPGQCLWEGCEPFQRQRFSFITHLQDKHCSKDALLAGLKQDEPGQAGSQKSSTKQPTVGGTSSTPRAQKAIVNHPSAALMALRRGSRNLVFRDFTDEKEGPITKHIRLTAALILKNIGKYSECGRRLLKRHENNLSVLAISNMEASSTLAKCLYELNFTVQSKEQEKDSEMLQ
Gene ID196528
Swiss protQ68CP9
SynonymsARID2; BAF200; p200
Calculated MW191kDa/197kDa
Observed MW250kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesLO2
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] ARID2 Polyclonal Antibody (A8601) }

Western blot - [KO Validated] ARID2 Polyclonal Antibody (A8601)

Western blot analysis of extracts of LO2 cells, using ARID2 antibody (A8601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] ARID2 Polyclonal Antibody (A8601) }

Western blot - [KO Validated] ARID2 Polyclonal Antibody (A8601)

Western blot analysis of extracts from normal (control) and ARID2 knockout (KO) HeLa cells, using ARID2 antibody (A8601) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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