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[KO Validated] ARHGEF1 Rabbit pAb (A4274)

Datasheet

ABclonal:Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274)
ABclonal:Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274)
ABclonal:Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274)

Overview

Product name[KO Validated] ARHGEF1 Rabbit pAb
Catalog No.A4274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
SequencePLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT
Gene ID9138
Swiss protQ92888
SynonymsARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5
Calculated MW98kDa/102kDa/104kDa/105kDa
Observed MW102kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat, LO2
Cellular locationCytoplasm, Membrane
ABclonal:Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274) }

Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274)

Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody (A4274) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274) }

Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274)

Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 antibody (A4274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274) }

Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (A4274)

Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 antibody (A4274) at dilution of 1:100 (40x lens).

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