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[KO Validated] ARHGDIA Rabbit pAb (A1214)

Publications (4) Datasheet

ABclonal:Western blot - [KO Validated] ARHGDIA Rabbit pAb (A1214)
ABclonal:Immunofluorescence - [KO Validated] ARHGDIA Rabbit pAb (A1214)

Overview

Product name[KO Validated] ARHGDIA Rabbit pAb
Catalog No.A1214
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
SequenceMAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD
Gene ID396
Swiss protP52565
SynonymsARHGDIA; GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1
Calculated MW18kDa/23kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T, HeLa, COS7, PC-12, Mouse lung, Mouse spleen, Mouse kidney
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - [KO Validated] ARHGDIA Rabbit pAb (A1214) }

Western blot - [KO Validated] ARHGDIA Rabbit pAb (A1214)

Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibody (A1214) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] ARHGDIA Rabbit pAb (A1214) }

Immunofluorescence - [KO Validated] ARHGDIA Rabbit pAb (A1214)

Immunofluorescence analysis of HeLa cells using ARHGDIA antibody (A1214). Blue: DAPI for nuclear staining.

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