Products > Catalog Antibodies > Monoclonal Antibodies

[KO Validated] APP Rabbit mAb (A17911)

Publications (2) Datasheet

ABclonal:[KO Validated] APP Rabbit mAb
ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (A17911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of extracts of various cell lines, using APP antibody (A17911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded mouse brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded rat brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded human brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911)

Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (A17911). Western blot was performed from the immunoprecipitate using APP antibody (A17911) at a dilition of 1:1000.

Overview

Product name[KO Validated] APP Rabbit mAb
Catalog No.A17911
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]
ImmunogenA synthesized peptide derived from human APP
SequenceEmail for sequence
Gene ID351
Swiss protP05067
SynonymsAAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; Amyloid beta A4; APP; Aβ42; preA4
Calculated MW86kDa
Observed MW100-140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, U-87MG, Mouse brain, Rat brain, 293T
Cellular locationMembrane, Single-pass type I membrane protein, clathrin-coated pit
Customer validation

IF(Rattus norvegicus)

WB(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911) }

Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (A17911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911) }

Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of extracts of various cell lines, using APP antibody (A17911) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911) }

Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded mouse brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911) }

Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded rat brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911) }

Immunohistochemistry - [KO Validated] APP Rabbit mAb (A17911)

Immunohistochemistry of paraffin-embedded human brain using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911) }

Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911)

Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911) }

Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (A17911). Western blot was performed from the immunoprecipitate using APP antibody (A17911) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A17911 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A17911? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order