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[KO Validated] APEX1 Rabbit pAb (A2587)

Datasheet

ABclonal:Western blot - [KO Validated] APEX1 Polyclonal Antibody (A2587)
ABclonal:Western blot - [KO Validated] APEX1 Polyclonal Antibody (A2587)
ABclonal:Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (A2587)
ABclonal:Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (A2587)
ABclonal:Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (A2587)

Overview

Product name[KO Validated] APEX1 Rabbit pAb
Catalog No.A2587
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
SequenceMPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL
Gene ID328
Swiss protP27695
SynonymsAPEX1; APE; APE1; APEN; APEX; APX; HAP1; REF1
Calculated MW35kDa
Observed MW39kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, HepG2
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Nucleus speckle, nucleolus
ABclonal:Western blot - [KO Validated] APEX1 Polyclonal Antibody (A2587) }

Western blot - [KO Validated] APEX1 Polyclonal Antibody (A2587)

Western blot analysis of extracts of various cell lines, using APEX1 antibody (A2587).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] APEX1 Polyclonal Antibody (A2587) }

Western blot - [KO Validated] APEX1 Polyclonal Antibody (A2587)

Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (A2587) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (A2587) }

Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (A2587)

Immunofluorescence analysis of HeLa cells using APEX1 antibody (A2587). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (A2587) }

Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (A2587)

Immunofluorescence analysis of A549 cells using APEX1 antibody (A2587).
ABclonal:Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (A2587) }

Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (A2587)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody (A2587). Western blot was performed from the immunoprecipitate using APEX1 antibody (A2587) at a dilition of 1:1000.

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