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[KO Validated] ANXA5 Rabbit pAb (A1728)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] ANXA5 Rabbit pAb (A1728)
ABclonal:Western blot - [KO Validated] ANXA5 Rabbit pAb (A1728)
ABclonal:Immunofluorescence - [KO Validated] ANXA5 Rabbit pAb (A1728)
Review
ABclonal: review for [KO Validated] ANXA5 Rabbit pAb(A1728)

Overview

Product name[KO Validated] ANXA5 Rabbit pAb
Catalog No.A1728
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ANXA5 (NP_001145.1).
SequenceMAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD
Gene ID308
Swiss protP08758
SynonymsANXA5; ANX5; ENX2; HEL-S-7; PP4; RPRGL3
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHepG2, HeLa, DU145, PC-3, Mouse lung
Cellular location
Customer validation

(Human prostate)

ABclonal:Western blot - [KO Validated] ANXA5 Rabbit pAb (A1728) }

Western blot - [KO Validated] ANXA5 Rabbit pAb (A1728)

Western blot analysis of extracts of various cell lines, using ANXA5 antibody (A1728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] ANXA5 Rabbit pAb (A1728) }

Western blot - [KO Validated] ANXA5 Rabbit pAb (A1728)

Western blot analysis of extracts from normal (control) and ANXA5 knockout (KO) 293T cells, using ANXA5 antibody (A1728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] ANXA5 Rabbit pAb (A1728) }

Immunofluorescence - [KO Validated] ANXA5 Rabbit pAb (A1728)

Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA5 Rabbit pAb (A1728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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