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[KO Validated] ANXA2 Rabbit pAb (A12397)

Datasheet

ABclonal:Western blot - [KO Validated] ANXA2 Polyclonal Antibody (A12397)
ABclonal:Western blot - [KO Validated] ANXA2 Polyclonal Antibody (A12397)
ABclonal:Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397)
ABclonal:Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397)
ABclonal:Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397)
ABclonal:Immunofluorescence - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Overview

Product name[KO Validated] ANXA2 Rabbit pAb
Catalog No.A12397
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human ANXA2 (NP_004030.1).
SequenceMSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Gene ID302
Swiss protP07355
SynonymsANXA2; ANX2; ANX2L4; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV
Calculated MW38kDa/40kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, HepG2, Mouse lung
Cellular locationMelanosome, Secreted, basement membrane, extracellular matrix, extracellular space
ABclonal:Western blot - [KO Validated] ANXA2 Polyclonal Antibody (A12397) }

Western blot - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Western blot analysis of extracts of various cell lines, using ANXA2 Antibody (A12397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] ANXA2 Polyclonal Antibody (A12397) }

Western blot - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Western blot analysis of extracts from normal (control) and ANXA2 knockout (KO) HeLa cells, using ANXA2 antibody (A12397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397) }

Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Immunohistochemistry of paraffin-embedded rat heart using ANXA2 antibody (A12397) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397) }

Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Immunohistochemistry of paraffin-embedded mouse liver using ANXA2 antibody (A12397) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397) }

Immunohistochemistry - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Immunohistochemistry of paraffin-embedded mouse heart using ANXA2 antibody (A12397) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] ANXA2 Polyclonal Antibody (A12397) }

Immunofluorescence - [KO Validated] ANXA2 Polyclonal Antibody (A12397)

Immunofluorescence analysis of A549 cells using ANXA2 antibody (A12397). Blue: DAPI for nuclear staining.

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