Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] ANXA1 Rabbit pAb (A1118)

KO/KDValidated

Review (1) Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] ANXA1 Rabbit pAb (A1118)

Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (A1118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] ANXA1 Rabbit pAb (A1118)

Western blot analysis of various lysates, using ANXA1 antibody (A1118) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunohistochemistry analysis of paraffin-embedded human spleen using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunohistochemistry analysis of paraffin-embedded human tonsil using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunofluorescence analysis of A-431 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunofluorescence analysis of A-549 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KO Validated] ANXA1 Rabbit pAb(A1118)

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Overview

Product name [KO Validated] ANXA1 Rabbit pAb
Catalog No. A1118
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
Sequence MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
Gene ID 301
Swiss prot P04083
Synonyms ANX1; LPC1; A1
Calculated MW 39kDa
Observed MW 38kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples C2C12, C6
Cellular location Apical cell membrane, Basolateral cell membrane, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Early endosome, Endosome membrane, Extracellular side, Lateral cell membrane, Membrane, Nucleus, Peripheral membrane protein, Secreted, cilium, exosome, extracellular space, phagocytic cup, secretory vesicle lumen
Customer validation

WB (Huh7.5.1,HepG2Homo sapiens, Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] ANXA1 Rabbit pAb images

ABclonal:Western blot - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Western blot - [KO Validated] ANXA1 Rabbit pAb (A1118)

Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (A1118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Western blot - [KO Validated] ANXA1 Rabbit pAb (A1118)

Western blot analysis of various lysates, using ANXA1 antibody (A1118) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Immunohistochemistry - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunohistochemistry analysis of paraffin-embedded human spleen using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Immunohistochemistry - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunohistochemistry analysis of paraffin-embedded human tonsil using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunofluorescence analysis of A-431 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunofluorescence analysis of A-549 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)}

Immunofluorescence - [KO Validated] ANXA1 Rabbit pAb (A1118)

Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on ANXA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ANXA1. (Distance between topics and target gene indicate popularity.) ANXA1

* Data provided by citexs.com, for reference only.

Publishing research using A1118? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (23)

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