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[KO Validated] ANXA1 Rabbit pAb (A1118)

Review (1) Datasheet

ABclonal:Western blot - [KO Validated] ANXA1 Polyclonal Antibody (A1118)
ABclonal:Western blot - [KO Validated] ANXA1 Polyclonal Antibody (A1118)
ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (A1118)
ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (A1118)
ABclonal:Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (A1118)
Review
ABclonal: review for [KO Validated] ANXA1 Rabbit pAb(A1118)

Overview

Product name[KO Validated] ANXA1 Rabbit pAb
Catalog No.A1118
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
SequenceMAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
Gene ID301
Swiss protP04083
SynonymsANXA1; ANX1; LPC1
Calculated MW38kDa
Observed MW38kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3
Cellular locationApical cell membrane, Basolateral cell membrane, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Early endosome, Endosome membrane, Extracellular side, Lateral cell membrane, Membrane, Nucleus, Peripheral membrane protein, Secreted, cilium, exosome, extracellular space, phagocytic cup, secretory vesicle lumen
Customer validation

(Huh7.5.1,HepG2)

ABclonal:Western blot - [KO Validated] ANXA1 Polyclonal Antibody (A1118) }

Western blot - [KO Validated] ANXA1 Polyclonal Antibody (A1118)

Western blot analysis of extracts of various cell lines, using ANXA1 antibody (A1118).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] ANXA1 Polyclonal Antibody (A1118) }

Western blot - [KO Validated] ANXA1 Polyclonal Antibody (A1118)

Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (A1118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (A1118) }

Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (A1118)

Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (A1118) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (A1118) }

Immunohistochemistry - [KO Validated] ANXA1 Polyclonal Antibody (A1118)

Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (A1118) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (A1118) }

Immunofluorescence - [KO Validated] ANXA1 Polyclonal Antibody (A1118)

Immunofluorescence analysis of HeLa cells using ANXA1 antibody (A1118). Blue: DAPI for nuclear staining.

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