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[KO Validated] AK2 Rabbit pAb (A6519)

Datasheet

ABclonal:Western blot - [KO Validated] AK2 Polyclonal Antibody (A6519)
ABclonal:Western blot - [KO Validated] AK2 Polyclonal Antibody (A6519)
ABclonal:Immunohistochemistry - [KO Validated] AK2 Polyclonal Antibody (A6519)

Overview

Product name[KO Validated] AK2 Rabbit pAb
Catalog No.A6519
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human AK2 (NP_001616.1).
SequenceMAPSVPAAEPEYPKGIRAVLLGPPGAGKGTQAPRLAENFCVCHLATGDMLRAMVASGSELGKKLKATMDAGKLVSDEMVVELIEKNLETPLCKNGFLLDGFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIPDSLLIRRITGRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKIRLQAYHTQTTPLIEYYRKRGIHSAIDASQTPDVVFASILAAFSKATC
Gene ID204
Swiss protP54819
SynonymsAK2; ADK2
Calculated MW14kDa/21kDa/22kDa/24kDa/25kDa/26kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHL-60, Mouse liver, Mouse kidney, Rat liver
Cellular locationMitochondrion intermembrane space
ABclonal:Western blot - [KO Validated] AK2 Polyclonal Antibody (A6519) }

Western blot - [KO Validated] AK2 Polyclonal Antibody (A6519)

Western blot analysis of extracts of various cell lines, using AK2 antibody (A6519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] AK2 Polyclonal Antibody (A6519) }

Western blot - [KO Validated] AK2 Polyclonal Antibody (A6519)

Western blot analysis of extracts from normal (control) and AK2 knockout (KO) 293T cells, using AK2 antibody (A6519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] AK2 Polyclonal Antibody (A6519) }

Immunohistochemistry - [KO Validated] AK2 Polyclonal Antibody (A6519)

Immunohistochemistry of paraffin-embedded human gastric cancer using AK2 antibody (A6519) at dilution of 1:100 (40x lens).

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