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[KO Validated] AIF Rabbit pAb (A2568)

Reviews (3)Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Western blot - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)
Review
ABclonal: review for [KO Validated] AIF Rabbit pAb(A2568)
Review
ABclonal: review for [KO Validated] AIF Rabbit pAb(A2568)
Review
ABclonal: review for [KO Validated] AIF Rabbit pAb(A2568)

Overview

Product name[KO Validated] AIF Rabbit pAb
Catalog No.A2568
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
SequenceFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED
Gene ID9131
Swiss protO95831
SynonymsAIF; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; AIFM1
Calculated MW26kDa/28kDa/35kDa/66kDa
Observed MW67kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSW620, 22Rv1, H460, PC-3, U-937, Mouse liver, Mouse kidney, Mouse heart
Cellular locationCytoplasm, Mitochondrion inner membrane, Mitochondrion intermembrane space, Nucleus, perinuclear region
Customer validation

(Eoma cell, 293T)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - [KO Validated] AIF Rabbit pAb (A2568) }

Western blot - [KO Validated] AIF Rabbit pAb (A2568)

Western blot analysis of extracts of various cell lines, using AIF antibody (A2568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] AIF Rabbit pAb (A2568) }

Western blot - [KO Validated] AIF Rabbit pAb (A2568)

Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (A2568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568) }

Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568)

Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] AIF Rabbit pAb (A2568) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568) }

Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568)

Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] AIF Rabbit pAb (A2568) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568) }

Immunohistochemistry - [KO Validated] AIF Rabbit pAb (A2568)

Immunohistochemistry of paraffin-embedded mouse stomach using [KO Validated] AIF Rabbit pAb (A2568) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568) }

Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)

Immunofluorescence analysis of C6 cells using AIF antibody (A2568) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568) }

Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)

Immunofluorescence analysis of L929 cells using AIF antibody (A2568) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568) }

Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)

Immunofluorescence analysis of mouse skeletal muscle using AIF antibody (A2568) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568) }

Immunofluorescence - [KO Validated] AIF Rabbit pAb (A2568)

Confocal immunofluorescence analysis of HeLa cells using AIFM1 antibody (A2568) at dilution of 1:200. Blue: DAPI for nuclear staining.

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