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[KO Validated] GRK2 Rabbit pAb (A1662)

Datasheet

ABclonal:Western blot - [KO Validated] GRK2 Rabbit pAb (A1662)
ABclonal:Western blot - [KO Validated] GRK2 Rabbit pAb (A1662)
ABclonal:Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662)
ABclonal:Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662)
ABclonal:Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662)

Overview

Product name[KO Validated] GRK2 Rabbit pAb
Catalog No.A1662
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2 (NP_001610.2).
SequenceLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL
Gene ID156
Swiss protP25098
SynonymsGRK2; ADRBK1; BARK1; BETA-ARK1
Calculated MW79kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHT-29, Mouse spleen, Mouse brain
Cellular locationCell membrane, Cytoplasm
ABclonal:Western blot - [KO Validated] GRK2 Rabbit pAb (A1662) }

Western blot - [KO Validated] GRK2 Rabbit pAb (A1662)

Western blot analysis of extracts of various cell lines, using GRK2 antibody (A1662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] GRK2 Rabbit pAb (A1662) }

Western blot - [KO Validated] GRK2 Rabbit pAb (A1662)

Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (A1662) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662) }

Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662)

Immunohistochemistry of paraffin-embedded rat brain using GRK2 Antibody (A1662) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662) }

Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662)

Immunohistochemistry of paraffin-embedded human esophageal cancer using GRK2 Antibody (A1662) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662) }

Immunohistochemistry - [KO Validated] GRK2 Rabbit pAb (A1662)

Immunohistochemistry of paraffin-embedded mouse kidney using GRK2 Antibody (A1662) at dilution of 1:200 (40x lens).

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