Products > Catalog Antibodies > Polyclonal Antibodies

KDM5A Rabbit pAb (A7238)

Datasheet

ABclonal:Western blot - KDM5A Rabbit pAb (A7238)

Western blot analysis of extracts of various cell lines, using KDM5A antibody (A7238) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.

ABclonal:Immunoprecipitation - KDM5A Rabbit pAb (A7238)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A antibody (A7238). Western blot was performed from the immunoprecipitate using KDM5A antibody (A7238) at a dilition of 1:500.

Overview

Product nameKDM5A Rabbit pAb
Catalog No.A7238
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
ImmunogenRecombinant protein of human KDM5A
SequenceEmail for sequence
Gene ID5927
Swiss protP29375
SynonymsKDM5A; RBBP-2; RBBP2; RBP2
Calculated MW186kDa/192kDa
Observed MW192kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, HeLa, MCF7
Cellular locationNucleus, nucleolus
ABclonal:Western blot - KDM5A Rabbit pAb (A7238) }

Western blot - KDM5A Rabbit pAb (A7238)

Western blot analysis of extracts of various cell lines, using KDM5A antibody (A7238) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
ABclonal:Immunoprecipitation - KDM5A Rabbit pAb (A7238) }

Immunoprecipitation - KDM5A Rabbit pAb (A7238)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A antibody (A7238). Western blot was performed from the immunoprecipitate using KDM5A antibody (A7238) at a dilition of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A7238 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order