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KDM4B/JMJD2B Rabbit pAb (A5824)

Reviews (3) Datasheet

ABclonal:Western blot - KDM4B/JMJD2B Rabbit pAb (A5824)

Western blot analysis of extracts of Mouse brain, using (A5824) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - KDM4B/JMJD2B Rabbit pAb (A5824)

Immunofluorescence analysis of U2OS cells using KDM4B/JMJD2B antibody (A5824). Blue: DAPI for nuclear staining.

Review
ABclonal: review for KDM4B/JMJD2B Rabbit pAb(A5824)
Review
ABclonal: review for KDM4B/JMJD2B Rabbit pAb(A5824)
Review
ABclonal: review for KDM4B/JMJD2B Rabbit pAb(A5824)

Overview

Product nameKDM4B/JMJD2B Rabbit pAb
Catalog No.A5824
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human KDM4B/JMJD2B (NP_055830.1).
SequenceMGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWNIGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSR
Gene ID23030
Swiss protO94953
SynonymsKDM4B; JMJD2B; TDRD14B
Calculated MW50kDa/121kDa
Observed MW150KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse brain
Cellular locationNucleus
Customer validation

(Human, HeLa)

ABclonal:Western blot - KDM4B/JMJD2B Rabbit pAb (A5824) }

Western blot - KDM4B/JMJD2B Rabbit pAb (A5824)

Western blot analysis of extracts of Mouse brain, using (A5824) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - KDM4B/JMJD2B Rabbit pAb (A5824) }

Immunofluorescence - KDM4B/JMJD2B Rabbit pAb (A5824)

Immunofluorescence analysis of U2OS cells using KDM4B/JMJD2B antibody (A5824). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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