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KDM3A Rabbit pAb (A11960)

Datasheet

ABclonal:Western blot - KDM3A Rabbit pAb (A11960)

Western blot analysis of extracts of Mouse testis, using KDM3A antibody (A11960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - KDM3A Rabbit pAb (A11960)

Immunohistochemistry of paraffin-embedded human liver cancer using KDM3A Rabbit pAb (A11960) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - KDM3A Rabbit pAb (A11960)

Immunofluorescence analysis of C6 cells using KDM3A Polyclonal Antibody (A11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KDM3A Rabbit pAb (A11960)

Immunofluorescence analysis of NIH-3T3 cells using KDM3A Polyclonal Antibody (A11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KDM3A Rabbit pAb (A11960)

Immunofluorescence analysis of U-2 OS cells using KDM3A Polyclonal Antibody (A11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameKDM3A Rabbit pAb
Catalog No.A11960
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-450 of human KDM3A (NP_060903.2).
SequenceLNTKSEALRTKPDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQENRLESVPQALTGLPKECLPTKASSKAELEIANPPELQKHLEHAPSPSDVS
Gene ID55818
Swiss protQ9Y4C1
SynonymsKDM3A; JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA
Calculated MW147kDa
Observed MW147KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse testis
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - KDM3A Rabbit pAb (A11960) }

Western blot - KDM3A Rabbit pAb (A11960)

Western blot analysis of extracts of Mouse testis, using KDM3A antibody (A11960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - KDM3A Rabbit pAb (A11960) }

Immunohistochemistry - KDM3A Rabbit pAb (A11960)

Immunohistochemistry of paraffin-embedded human liver cancer using KDM3A Rabbit pAb (A11960) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - KDM3A Rabbit pAb (A11960) }

Immunofluorescence - KDM3A Rabbit pAb (A11960)

Immunofluorescence analysis of C6 cells using KDM3A Polyclonal Antibody (A11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KDM3A Rabbit pAb (A11960) }

Immunofluorescence - KDM3A Rabbit pAb (A11960)

Immunofluorescence analysis of NIH-3T3 cells using KDM3A Polyclonal Antibody (A11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KDM3A Rabbit pAb (A11960) }

Immunofluorescence - KDM3A Rabbit pAb (A11960)

Immunofluorescence analysis of U-2 OS cells using KDM3A Polyclonal Antibody (A11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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