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KAT7 Rabbit pAb (A5823)

Publication (1) Datasheet

ABclonal:Western blot - KAT7 Rabbit pAb (A5823)

Western blot analysis of extracts of various cell lines, using KAT7 antibody (A5823) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded rat spleen using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded rat kidney using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded human colon carcinoma using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded human breast cancer using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded mouse liver using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - KAT7 Rabbit pAb (A5823)

Immunofluorescence analysis of HeLa cells using KAT7 antibody (A5823) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameKAT7 Rabbit pAb
Catalog No.A5823
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 55-193 of human KAT7 (NP_008998.1).
SequenceDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSL
Gene ID11143
Swiss protO95251
SynonymsKAT7; HBO1; HBOA; MYST2; ZC2HC7
Calculated MW51kDa/55kDa/58kDa/66kDa/70kDa
Observed MW63kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, HepG2, 293T, HeLa
Cellular locationNucleus, nucleoplasm
Customer validation

WB(Danio rerio)

ABclonal:Western blot - KAT7 Rabbit pAb (A5823) }

Western blot - KAT7 Rabbit pAb (A5823)

Western blot analysis of extracts of various cell lines, using KAT7 antibody (A5823) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823) }

Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded rat spleen using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823) }

Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded rat kidney using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823) }

Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded human colon carcinoma using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823) }

Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded human breast cancer using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - KAT7 Rabbit pAb (A5823) }

Immunohistochemistry - KAT7 Rabbit pAb (A5823)

Immunohistochemistry of paraffin-embedded mouse liver using KAT7 Rabbit pAb (A5823) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - KAT7 Rabbit pAb (A5823) }

Immunofluorescence - KAT7 Rabbit pAb (A5823)

Immunofluorescence analysis of HeLa cells using KAT7 antibody (A5823) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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