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JNK1 Rabbit pAb (A0288)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - JNK1 Rabbit pAb (A0288)
ABclonal:Immunohistochemistry - JNK1 Rabbit pAb (A0288)
ABclonal:Immunofluorescence - JNK1 Rabbit pAb (A0288)
ABclonal: review for JNK1 Rabbit pAb(A0288)


Product nameJNK1 Rabbit pAb
Catalog No.A0288
Host speciesRabbit
Purification methodAffinity purification
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 245-345 of human JNK1 (NP_620635.1).
Gene ID5599
Swiss protP45983
SynonymsMAPK8; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c
Calculated MW35kDa/44kDa/48kDa
Observed MW50kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, HeLa, BT-474, Mouse liver
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse heart tissue)

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IP(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - JNK1 Rabbit pAb (A0288) }

Western blot - JNK1 Rabbit pAb (A0288)

Western blot analysis of extracts of various cell lines, using JNK1 antibody (A0288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - JNK1 Rabbit pAb (A0288) }

Immunohistochemistry - JNK1 Rabbit pAb (A0288)

Immunohistochemistry of paraffin-embedded human lung cancer using JNK1 antibody (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - JNK1 Rabbit pAb (A0288) }

Immunofluorescence - JNK1 Rabbit pAb (A0288)

Immunofluorescence analysis of HeLa cells using JNK1 antibody (A0288) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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