Products > Catalog Antibodies > Polyclonal Antibodies

JNK1 Polyclonal Antibody (A0288)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - JNK1 Polyclonal Antibody (A0288)
ABclonal:Immunohistochemistry - JNK1 Polyclonal Antibody (A0288)
ABclonal:Immunofluorescence - JNK1 Polyclonal Antibody (A0288)
Review
ABclonal: review for JNK1 Polyclonal Antibody(A0288)

Overview

Product nameJNK1 Polyclonal Antibody
Catalog No.A0288
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 245-345 of human JNK1 (NP_620635.1).
SequenceCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDER
Gene ID5599
Swiss protP45983
SynonymsMAPK8; JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c; mitogen-activated protein kinase 8
Calculated MW35kDa/44kDa/48kDa
Observed MW50kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, HeLa, BT-474, Mouse liver
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human, Rat, Mouse)

ABclonal:Western blot - JNK1 Polyclonal Antibody (A0288) }

Western blot - JNK1 Polyclonal Antibody (A0288)

Western blot analysis of extracts of various cell lines, using JNK1 antibody (A0288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - JNK1 Polyclonal Antibody (A0288) }

Immunohistochemistry - JNK1 Polyclonal Antibody (A0288)

Immunohistochemistry of paraffin-embedded human lung cancer using JNK1 antibody (A0288) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - JNK1 Polyclonal Antibody (A0288) }

Immunofluorescence - JNK1 Polyclonal Antibody (A0288)

Immunofluorescence analysis of HeLa cells using JNK1 antibody (A0288) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Submit an review for A0288

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A0288 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A0288? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order