Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)

Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (A0076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)

Western blot analysis of extracts of Mouse lung, using Integrin beta 3 (ITGB3/CD61) antibody (A0076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name Integrin beta 3 (ITGB3/CD61) Rabbit pAb
Catalog No. A0076
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 609-788 of human ITGB3 (NP_000203.2).
Sequence CGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGALHDENTCNRYCRDEIESVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT
Gene ID 3690
Swiss prot P05106
Synonyms GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24; Integrin beta 3 (ITGB3/CD61)
Calculated MW 87kDa
Observed MW 97kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, U-87MG, Mouse lung
Cellular location Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane

Documents

Certificate of Compliance

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Lot number

Research Area

Integrin beta 3 (ITGB3/CD61) Rabbit pAb images

ABclonal:Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)}

Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)

Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (A0076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)}

Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit pAb (A0076)

Western blot analysis of extracts of Mouse lung, using Integrin beta 3 (ITGB3/CD61) antibody (A0076) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ITGB3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ITGB3. (Distance between topics and target gene indicate popularity.) ITGB3

* Data provided by citexs.com, for reference only.

Publishing research using A0076? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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