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Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Integrin beta 3 (ITGB3/CD61) Rabbit mAb
ABclonal:Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) (ITGB3/CD61) antibody (A19073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Immunohistochemistry of paraffin-embedded rat bone marrow using Integrin beta 3 (ITGB3/CD61) (ITGB3/CD61) Rabbit mAb (A19073) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Immunohistochemistry of paraffin-embedded mouse bone marrow using Integrin beta 3 (ITGB3/CD61) (ITGB3/CD61) Rabbit mAb (A19073) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug Integrin beta 3 (ITGB3/CD61) antibody (A19073). Western blot was performed from the immunoprecipitate using Integrin beta 3 (ITGB3/CD61) antibody (A19073) at a dilition of 1:1000.

ABclonal: - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Flow cytometry: HEL cells were stained with Rabbit IgG isotype control (AC042, 2.5 ug/ml, blue line) or Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073, 2.5 ug/ml orange line), followed by FITC conjugated goat anti-rabbit pAb (1:200 dilution) staining. Non-fluorescently stained HEL cells were used as blank control (red line).

ABclonal: - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Flow cytometry: U937 cells were stained with Rabbit IgG isotype control (AC042, 2.5 ug/ml, blue line) or Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073, 2.5 ug/ml orange line), followed by FITC conjugated goat anti-rabbit pAb (1:200 dilution). Non-fluorescently stained U937 cells were used as blank control (red line).

Overview

Product nameIntegrin beta 3 (ITGB3/CD61) Rabbit mAb
Catalog No.A19073
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 689-788 of human Integrin beta 3 (ITGB3/CD61) (P05106).
SequenceCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT
Gene ID3690
Swiss protP05106
SynonymsBDPLT16; BDPLT2; CD61; GP3A; GPIIIa; GT; Integrin beta 3; ITGB3
Calculated MW110kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IP 1:50 - 1:200
  • FC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    Flow Cytometry    
Positive samplesU-87MG, HUVEC, Mouse lung
Cellular locationCell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane
Customer validation

IF(Mus musculus)

WB(Homo sapiens)

ABclonal:Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)}

Western blot - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) (ITGB3/CD61) antibody (A19073) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)}

Immunohistochemistry - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Immunohistochemistry of paraffin-embedded rat bone marrow using Integrin beta 3 (ITGB3/CD61) (ITGB3/CD61) Rabbit mAb (A19073) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)}

Immunohistochemistry - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Immunohistochemistry of paraffin-embedded mouse bone marrow using Integrin beta 3 (ITGB3/CD61) (ITGB3/CD61) Rabbit mAb (A19073) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)}

Immunoprecipitation - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug Integrin beta 3 (ITGB3/CD61) antibody (A19073). Western blot was performed from the immunoprecipitate using Integrin beta 3 (ITGB3/CD61) antibody (A19073) at a dilition of 1:1000.
ABclonal: - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)}

- Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Flow cytometry: HEL cells were stained with Rabbit IgG isotype control (AC042, 2.5 ug/ml, blue line) or Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073, 2.5 ug/ml orange line), followed by FITC conjugated goat anti-rabbit pAb (1:200 dilution) staining. Non-fluorescently stained HEL cells were used as blank control (red line).
ABclonal: - Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)}

- Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073)

Flow cytometry: U937 cells were stained with Rabbit IgG isotype control (AC042, 2.5 ug/ml, blue line) or Integrin beta 3 (ITGB3/CD61) Rabbit mAb (A19073, 2.5 ug/ml orange line), followed by FITC conjugated goat anti-rabbit pAb (1:200 dilution). Non-fluorescently stained U937 cells were used as blank control (red line).

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