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Insulin Rabbit pAb (A2090)

Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Insulin Rabbit pAb (A2090)

Western blot analysis of extracts of Rat pancreas, using Insulin antibody (A2090) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Insulin Rabbit pAb (A2090)

Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of Rat pancreas using Insulin Rabbit pAb (A2090) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of Mouse pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameInsulin Rabbit pAb
Catalog No.A2090
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-110 of human INS (NP_000198.1).
SequenceFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN
Gene ID3630
Swiss protP01308
SynonymsIDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10; INS; Insulin; insulin
Calculated MW11kDa/21kDa
Observed MW10KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat pancreas
Cellular locationSecreted
Customer validation

IHC(Mus musculus, Homo sapiens)

IF(Mus musculus)

WB(Mus musculus)

ABclonal:Western blot - Insulin Rabbit pAb (A2090) }

Western blot - Insulin Rabbit pAb (A2090)

Western blot analysis of extracts of Rat pancreas, using Insulin antibody (A2090) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Insulin Rabbit pAb (A2090) }

Immunohistochemistry - Insulin Rabbit pAb (A2090)

Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090) }

Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of Rat pancreas using Insulin Rabbit pAb (A2090) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090) }

Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of Mouse pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100. Blue: DAPI for nuclear staining.

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