Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Insulin Rabbit pAb (A2090)

Publications (8) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Insulin Rabbit pAb (A2090)

Western blot analysis of various lysates, using Insulin antibody (A2090) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Insulin Rabbit pAb (A2090)

Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of human pancreas cells using Insulin Rabbit pAb (A2090) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of mouse pancreas cells using Insulin Rabbit pAb (A2090) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of rat pancreas cells using Insulin Rabbit pAb (A2090) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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    Overview

    Product nameInsulin Rabbit pAb
    Catalog No.A2090
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-110 of human INS (NP_000198.1).
    SequenceFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN
    Gene ID3630
    Swiss protP01308
    SynonymsIDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
    Calculated MW12kDa
    Observed MW6kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:1000
    • IHC-P 1:50 - 1:200
    • IF/ICC 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
    Positive samplesMouse pancreas, Rat pancreas
    Cellular locationSecreted
    Customer validation

    IHC(Mus musculus, Homo sapiens)

    IF(Mus musculus, Homo sapiens)

    WB(Mus musculus)

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    Insulin Rabbit pAb images

    ABclonal:Western blot - Insulin Rabbit pAb (A2090)}

    Western blot - Insulin Rabbit pAb (A2090)

    Western blot analysis of various lysates, using Insulin antibody (A2090) at 1:600 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Insulin Rabbit pAb (A2090)}

    Immunohistochemistry - Insulin Rabbit pAb (A2090)

    Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)}

    Immunofluorescence - Insulin Rabbit pAb (A2090)

    Immunofluorescence analysis of human pancreas cells using Insulin Rabbit pAb (A2090) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)}

    Immunofluorescence - Insulin Rabbit pAb (A2090)

    Immunofluorescence analysis of mouse pancreas cells using Insulin Rabbit pAb (A2090) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)}

    Immunofluorescence - Insulin Rabbit pAb (A2090)

    Immunofluorescence analysis of rat pancreas cells using Insulin Rabbit pAb (A2090) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on INS. (Numbers indicate number of published articles where both targets appear.)

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    Related Research Area

    Based on existing publications, the following research topics are related to INS. (Distance between topics and target gene indicate popularity.) INS

    * Data provided by citexs.com, for reference only.

    Publishing research using A2090? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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