Western blot analysis of extracts of mouse pancreas, using Insulin antibody (A2090) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteInsulin: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin antibody (A2090) at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreatic islet using Insulin antibody (A2090) at dilution of 1:50 (40x lens).
Immunofluorescence analysis of Rat pancreas using Insulin Rabbit pAb (A2090) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | Insulin Rabbit pAb |
|---|---|
| Catalog No. | A2090 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS (NP_000198.1). |
|---|---|
| Sequence | MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN |
| Gene ID | 3630 |
| Swiss prot | P01308 |
| Synonyms | IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10; INS; Insulin; insulin |
| Calculated MW | 11kDa/21kDa |
| Observed MW | 12kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | Mouse pancreas |
| Cellular location | Secreted |
| Customer validation | IHC(Homo sapiens, Mus musculus) |
* For research use only. Not for therapeutic or diagnostic purposes.
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