Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Insulin Rabbit pAb (A2090)

Publications (10) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Insulin Rabbit pAb (A2090)

Western blot analysis of various lysates, using Insulin Rabbit pAb (A2090) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Insulin Rabbit pAb (A2090)

Immunohistochemistry analysis of Insulin in paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of paraffin-embedded Rat pancreas using Insulin Rabbit pAb(A2090) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of paraffin-embedded Mouse pancreas using Insulin Rabbit pAb(A2090) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of paraffin-embedded Human pancreas using Insulin Rabbit pAb(A2090) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

You may also interested in:

Overview

Product name Insulin Rabbit pAb
Catalog No. A2090
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS (NP_000198.1).
Sequence MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN
Gene ID 3630
Swiss prot P01308
Synonyms IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10; Insulin
Calculated MW 12kDa
Observed MW

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Moouse pancreas, Rat pancreas
Cellular location Secreted
Customer validation

IHC (Mus musculus, Homo sapiens)

IF (Mus musculus, Homo sapiens)

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Insulin Rabbit pAb images

ABclonal:Western blot - Insulin Rabbit pAb (A2090)}

Western blot - Insulin Rabbit pAb (A2090)

Western blot analysis of various lysates, using Insulin Rabbit pAb (A2090) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Insulin Rabbit pAb (A2090)}

Immunohistochemistry - Insulin Rabbit pAb (A2090)

Immunohistochemistry analysis of Insulin in paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb (A2090) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)}

Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of paraffin-embedded Rat pancreas using Insulin Rabbit pAb(A2090) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)}

Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of paraffin-embedded Mouse pancreas using Insulin Rabbit pAb(A2090) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Insulin Rabbit pAb (A2090)}

Immunofluorescence - Insulin Rabbit pAb (A2090)

Immunofluorescence analysis of paraffin-embedded Human pancreas using Insulin Rabbit pAb(A2090) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Inquire About This Product

Submit your question about A2090 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on INS. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to INS. (Distance between topics and target gene indicate popularity.) INS

* Data provided by citexs.com, for reference only.

Publishing research using A2090? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order