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IRS1 Rabbit pAb (A0245)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of various cell lines, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of 293T cells, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry of paraffin-embedded human lung cancer using IRS1 Rabbit pAb (A0245) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry of paraffin-embedded mouse spinal cord using IRS1 Rabbit pAb (A0245) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry of paraffin-embedded rat brain using IRS1 Rabbit pAb (A0245) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for IRS1 Rabbit pAb(A0245)

Overview

Product nameIRS1 Rabbit pAb
Catalog No.A0245
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
ImmunogenRecombinant protein of human IRS1
SequenceEmail for sequence
Gene ID3667
Swiss protP35568
SynonymsHIRS-1; IRS1
Calculated MW131kDa
Observed MW180KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse liver, Rat liver
Cellular location
Customer validation

(mouse stomach cells)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - IRS1 Rabbit pAb (A0245) }

Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of various cell lines, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - IRS1 Rabbit pAb (A0245) }

Western blot - IRS1 Rabbit pAb (A0245)

Western blot analysis of extracts of 293T cells, using IRS1 antibody (A0245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245) }

Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry of paraffin-embedded human lung cancer using IRS1 Rabbit pAb (A0245) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245) }

Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry of paraffin-embedded mouse spinal cord using IRS1 Rabbit pAb (A0245) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IRS1 Rabbit pAb (A0245) }

Immunohistochemistry - IRS1 Rabbit pAb (A0245)

Immunohistochemistry of paraffin-embedded rat brain using IRS1 Rabbit pAb (A0245) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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