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IRF3 Rabbit pAb (A11373)

Publications (3) Datasheet

ABclonal:Western blot - IRF3 Rabbit pAb (A11373)

Western blot analysis of extracts of various cell lines, using IRF3 antibody (A11373) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded rat lung using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded human liver cancer using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded mouse kidney using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - IRF3 Rabbit pAb (A11373)

Immunofluorescence analysis of THP-1 cells using IRF3 Rabbit pAb (A11373) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IRF3 Rabbit pAb (A11373)

Immunofluorescence analysis of U-2 OS cells using IRF3 Rabbit pAb (A11373) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameIRF3 Rabbit pAb
Catalog No.A11373
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
SequenceMGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSY
Gene ID3661
Swiss protQ14653
SynonymsIIAE7; IRF3
Calculated MW12kDa/16kDa/33kDa/47kDa/49kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, HT-29
Cellular locationCytoplasm, Nucleus
Customer validation

IF(Homo sapiens)

WB(Homo sapiens)

ABclonal:Western blot - IRF3 Rabbit pAb (A11373) }

Western blot - IRF3 Rabbit pAb (A11373)

Western blot analysis of extracts of various cell lines, using IRF3 antibody (A11373) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373) }

Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded rat lung using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373) }

Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded human liver cancer using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373) }

Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11373) }

Immunohistochemistry - IRF3 Rabbit pAb (A11373)

Immunohistochemistry of paraffin-embedded mouse kidney using IRF3 antibody (A11373) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - IRF3 Rabbit pAb (A11373) }

Immunofluorescence - IRF3 Rabbit pAb (A11373)

Immunofluorescence analysis of THP-1 cells using IRF3 Rabbit pAb (A11373) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IRF3 Rabbit pAb (A11373) }

Immunofluorescence - IRF3 Rabbit pAb (A11373)

Immunofluorescence analysis of U-2 OS cells using IRF3 Rabbit pAb (A11373) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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