Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

IP3R Rabbit pAb (A7905)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - IP3R Rabbit pAb (A7905)

Western blot analysis of various lysates using IP3R Rabbit pAb (A7905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name IP3R Rabbit pAb
Catalog No. A7905
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an intracellular receptor for inositol 1, 4, 5-trisphosphate. Upon stimulation by inositol 1, 4, 5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1812-1911 of human IP3R (XP_011531985.1).
Sequence ASNLVIDLIMNASSDRVFHESILLAIALLEGGNTTIQHSFFCRLTEDKKSEKFFKVFYDRMKVAQQEIKATVTVNTSDLGNKKKDDEVDRDAPSRKKAKE
Gene ID 3708
Swiss prot Q14643
Synonyms ACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94
Calculated MW 314kDa
Observed MW 314kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, Mouse brain
Cellular location Endoplasmic reticulum membrane, Multi-pass membrane protein
Customer validation

WB (Homo sapiens, Mus musculus, Sus scrofa)

IHC (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

IP3R Rabbit pAb images

ABclonal:Western blot - IP3R Rabbit pAb (A7905)}

Western blot - IP3R Rabbit pAb (A7905)

Western blot analysis of various lysates using IP3R Rabbit pAb (A7905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ITPR1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ITPR1. (Distance between topics and target gene indicate popularity.) ITPR1

* Data provided by citexs.com, for reference only.

Publishing research using A7905? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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