Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

INPP4B Rabbit pAb (A16461)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - INPP4B Rabbit pAb (A16461)

Western blot analysis of various lysates using INPP4B Rabbit pAb (A16461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 150s.

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Overview

Product name INPP4B Rabbit pAb
Catalog No. A16461
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

INPP4B encodes the inositol polyphosphate 4-phosphatase type II, one of the enzymes involved in phosphatidylinositol signaling pathways. This enzyme removes the phosphate group at position 4 of the inositol ring from inositol 3, 4-bisphosphate. There is limited data to suggest that the human type II enzyme is subject to alternative splicing, as has been established for the type I enzyme.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 380-600 of human INPP4B (NP_003857.2).
Sequence GLRKLLHRFETERRNTGYQFIYYSPENTAKAKEVLSNINQLQPLIATHADLLLNSASQHSPDSLKNSLKMLSEKTELFVHAFKDQLVRSALLALYTARPGGILKKPPSPKSSTEESSPQDQPPVMRGQDSIPHHSDYDEEEWDRVWANVGKSLNCIIAMVDKLIERDGGSEGSGGNNDGEKEPSLTDAIPSHPREDWYEQLYPLILTLKDCMGEVVNRAKQ
Gene ID 8821
Swiss prot O15327
Synonyms INPP4B
Calculated MW 105kDa
Observed MW 98kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples U-87MG, A375
Cellular location cytoplasm, cytosol

Documents

Certificate of Compliance

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Lot number

INPP4B Rabbit pAb images

ABclonal:Western blot - INPP4B Rabbit pAb (A16461)}

Western blot - INPP4B Rabbit pAb (A16461)

Western blot analysis of various lysates using INPP4B Rabbit pAb (A16461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 150s.

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Related Targets

Based on existing publications, the following genes are closely related to research on INPP4B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to INPP4B. (Distance between topics and target gene indicate popularity.) INPP4B

* Data provided by citexs.com, for reference only.

Publishing research using A16461? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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