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IL6 Rabbit pAb (A0286)

Reviews (4)Publications (31) Datasheet

ABclonal:IL6 Rabbit pAb
ABclonal:Western blot - IL6 Rabbit pAb (A0286)

Western blot analysis of extracts of various cell lines, using IL6 antibody (A0286) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IL6 Rabbit pAb (A0286)

Immunohistochemistry of paraffin-embedded rat spleen using IL6 Rabbit pAb (A0286) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - IL6 Rabbit pAb (A0286)

Immunohistochemistry of paraffin-embedded mouse spleen using IL6 Rabbit pAb (A0286) at dilution of 1:100 (40x lens).

Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)

Overview

Product nameIL6 Rabbit pAb
Catalog No.A0286
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6 (NP_000591.1).
SequenceVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
Gene ID3569
Swiss protP05231
SynonymsIL6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Calculated MW23kDa
Observed MW26KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549, NCI-H460, THP-1
Cellular locationSecreted
Customer validation

(Mouse embryonal fibroblast cell, Human colon cancer cell, Mouse skeletal muscle)

WB(Oryctolagus cuniculus, Homo sapiens, Rattus norvegicus, Mus musculus, Cynoglossus semilaevis)

IHC(Mus musculus, Homo sapiens)

IHC(Mus musculus)

ELISA(Mus musculus)

WB(Mus musculus)

IF(Mus musculus)

IF(Homo sapiens,Mus musculus)

ABclonal:Western blot - IL6 Rabbit pAb (A0286) }

Western blot - IL6 Rabbit pAb (A0286)

Western blot analysis of extracts of various cell lines, using IL6 antibody (A0286) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IL6 Rabbit pAb (A0286) }

Immunohistochemistry - IL6 Rabbit pAb (A0286)

Immunohistochemistry of paraffin-embedded rat spleen using IL6 Rabbit pAb (A0286) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - IL6 Rabbit pAb (A0286) }

Immunohistochemistry - IL6 Rabbit pAb (A0286)

Immunohistochemistry of paraffin-embedded mouse spleen using IL6 Rabbit pAb (A0286) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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